Isolation of Monocytes from Bovine Blood for Purposes of Culture of Dendritic Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43916589" target="_blank" >RIV/62156489:43210/19:43916589 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/19:N0000133
Result on the web
<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430" target="_blank" >http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.427-430" target="_blank" >10.15414/jmbfs.2019.9.special.427-430</a>
Alternative languages
Result language
angličtina
Original language name
Isolation of Monocytes from Bovine Blood for Purposes of Culture of Dendritic Cells
Original language description
Monocytes comprise a population of mononuclear leukocytes. In this article, we compare methods for isolating monocytes and also their cultivation into dendritic cells. Bovine blood collected from the vena jugularis externa was used for the experiment. This blood was used for isolating monocytes based on density gradient of OptiPrep or Histopaque solutions. The results indicated that the more efficient method is isolation based on density gradient of the Histopaque solution and subsequent magnetic separation of the cells. The monocyte population was further cultivated for 72 h with IL-4 and GM-CSF. The cells were evaluated under a light microscope, and it was apparent that CD14-positive cells transformed to dendritic cells. The second method using the Histopaque solution and magnetic separation was more efficient for the subsequent culture of dendritic cells.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/QK1910212" target="_blank" >QK1910212: Modern methods of diagnosis, therapy and prevention of mastitis caused by Streptococcus uberis as a tool for designing targeted control programs in cows</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiology, Biotechnology and Food Science
ISSN
1338-5178
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
Special Issue
Country of publishing house
SK - SLOVAKIA
Number of pages
4
Pages from-to
427-430
UT code for WoS article
000500544500014
EID of the result in the Scopus database
2-s2.0-85083482487