Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875609" target="_blank" >RIV/60162694:G44__/16:43875609 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/16:43909873 RIV/00216275:25310/16:39902006
Result on the web
<a href="http://electrochemsci.org/papers/vol11/110604840.pdf" target="_blank" >http://electrochemsci.org/papers/vol11/110604840.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.20964/2016.06.39" target="_blank" >10.20964/2016.06.39</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles
Original language description
Acetylcholinesterase (AChE) is an enzyme which terminates action of neurotransmitter acetylcholine in cholinergic system. Activity of enzyme is commonly assayed by Ellman's method which has significant disadvantages like instability of reagents. Inability to use enzyme from the mixture repeatedly is another disadvantage. Therefore, we developed process using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) to immobilize AChE on the surface of magnetic particles, which enable to separate enzyme and use it repeatedly. To determinate activity of enzyme, we optimized square wave voltammetry (SWV) method where screen-printed sensor with Prussian blue (PB) modified working electrode was performed. We report limit of detection equal 8.1 mu M for a tested inhibitor of AChE tacrine. Comparing to the standard Ellman's test, the immobilized enzyme has limited sensitivity to interferences caused by organic solvents. The proposed method is readily to practical application.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Electrochemical Science
ISSN
1452-3981
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
6
Country of publishing house
CS - SERBIA AND MONTENEGRO
Number of pages
10
Pages from-to
4840-4849
UT code for WoS article
000378559300050
EID of the result in the Scopus database
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