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HI-6 treatment does not reactivate sarin inhibited acetylcholinesterase activity in dog brain when administered in human therapeutical dose 30 minutes after the poisoning

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875648" target="_blank" >RIV/60162694:G44__/16:43875648 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11150/16:10338322 RIV/00179906:_____/16:10338322

  • Result on the web

    <a href="http://mmsl.cz/viCMS/soubory/pdf/MMSL_2016_1_1_WWW.pdf" target="_blank" >http://mmsl.cz/viCMS/soubory/pdf/MMSL_2016_1_1_WWW.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    HI-6 treatment does not reactivate sarin inhibited acetylcholinesterase activity in dog brain when administered in human therapeutical dose 30 minutes after the poisoning

  • Original language description

    The aim of our study was to determine and compare the activity of acetylcholinesterase (AChE) in different parts of dog brain after the exposure to nerve agent sarin with or without HI-6 oxime treatment. Material and methods: Before intoxication, beagle dogs were intravenously anaesthetized and premedicated with atropine sulphate (0.01 mg/kg). Three xperimental groups were established - control, sarin (0.03 mg/kg, intramuscularly, 5 min after anaesthesia onset), and sarin + HI-6 dichloride (11.4 mg/kg, intramuscularly, 30 min after sarin poisoning). Brain (amygdaloid body, head of caudate nucleus, somatosensory cortex, Amon's horn of hippocampus, ypothalamus, brain stem ventral respiratory group, and medial nuclei of thalamus) samples were taken 4 h after sarin administration. AChE activity was detected by histochemistry using the Karnovsky-Roots method and computer image analysis. Results: Sarin poisoning decreased AChE activity in all selected brain areas. HI-6 did not affect this outcome. Conclusion: HI-6 does not reactivate brain AChE in dogs when administered 30 min after sarin poisoning.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FP - Other medical fields

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Military Medical Science Letters

  • ISSN

    0372-7025

  • e-ISSN

  • Volume of the periodical

    85

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    6

  • Pages from-to

    2-7

  • UT code for WoS article

  • EID of the result in the Scopus database