Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889442" target="_blank" >RIV/60162694:G44__/18:43889442 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/18:10380165 RIV/00216208:11160/18:10380165

Result on the web

<a href="https://link.springer.com/content/pdf/10.1007%2Fs12272-017-1000-4.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs12272-017-1000-4.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12272-017-1000-4" target="_blank" >10.1007/s12272-017-1000-4</a>

Result language

angličtina

Original language name

Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity

Original language description

Fifteen Amaryllidaceae alkaloids (1-15) of various structural types were isolated by standard chromatographic methods from fresh bulbs of Narcissus poeticus cv. Pink Parasol. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic analyses, and by comparison with literature data. Narcipavline (5) and narcikachnine (6) are reported here for the first time. In their structure are combined two basic structural types of Amaryllidaceae alkaloids (galanthamine- and galanthindole-structural types), which represent a new structural type of these compounds. Alkaloids isolated in sufficient amounts were evaluated for their human erythrocytic acetylcholinesterase, and human serum butyrylcholinesterase (HuBuChE) inhibition activity using Ellman&apos;s method. Z-Gly-Pro-p-nitroanilide was used as substrate in the prolyl oligopeptidase (POP) assay. Untested alkaloids were also screened for their cytotoxic activity against a small panel of human cancer cells, which spanned cell lines from different tissue types. In parallel, MRC-5 human fibroblasts were employed to determine overall toxicity against noncancerous cells. Some compounds were evaluated for their antiprotozoal activity. The newly isolated alkaloid narcipavline (5) showed interesting HuBuChE inhibition activity (IC50 = 24.4 +/- 1.2 A mu M), and norlycoramine (11) demonstrated promising POP inhibition (IC50 = 0.21 +/- 0.01 mM).

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Pharmacal Research

ISSN

0253-6269

e-ISSN

—

Volume of the periodical

41

Issue of the periodical within the volume

2

Country of publishing house

KR - KOREA, REPUBLIC OF

Number of pages

11

Pages from-to

208-218

UT code for WoS article

000424689700007

EID of the result in the Scopus database

2-s2.0-85038083938