Lack of Zika virus antibody response in confirmed patients in non-endemic countries

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889468" target="_blank" >RIV/60162694:G44__/18:43889468 - isvavai.cz</a>

Result on the web

<a href="http://www.journalofclinicalvirology.com/article/S1386-6532(17)30346-3/pdf" target="_blank" >http://www.journalofclinicalvirology.com/article/S1386-6532(17)30346-3/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcv.2017.12.007" target="_blank" >10.1016/j.jcv.2017.12.007</a>

Result language

angličtina

Original language name

Lack of Zika virus antibody response in confirmed patients in non-endemic countries

Original language description

Zika virus (ZIKV) has spread in the last 2 years throughout America and South-Eastern Asia causing a widespread epidemic. Detection of ZIKV RNA in body fluids confirms ZIKV infection, however ZIKV antibody testing is much more complex due to possible cross-reactivity with closely related flaviviruses. From December 2015 to February 2017, 401 patients from eight reference laboratories in the Czech Republic, Israel, Italy, the Netherlands, Romania, Slovenia, and the United Kingdom had been confirmed for ZIKV infection by detection of ZIKV RNA in body fluids. Of these 401 patients, 148 were negative for ZIKV directed against IgM and IgG in serum collected at the time of PCR-positivity as tested by ELISA (7 laboratories, Euroimmun, Lübeck, Germany) or IFA and ELISA (2 laboratories, Euroimmun, Lübeck, Germany). For 80 of these 148 seronegative confirmed patients a second, follow-up serum sample was available. Altogether, 5 of these 80 patients remained without seroconversion in consecutive samples for ZIKV antibodies tested by ELISA and virus neutralization (VNT). The acute samples of these 5 patients were re-extracted and retested from original material which confirmed the presence of ZIKV RNA. Material from patients 1 and 2 were sequenced. Ideally, each of the samples from the 5 patients would also have been tested in at least one of the other laboratories, but because of insufficient clinical material, this wasn’t possible. Most importantly, none of the sero-negative patients had any indication of immune-deficiency. Two patients were pregnant.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

—

Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Clinical Virology

ISSN

1386-6532

e-ISSN

—

Volume of the periodical

99-100

Issue of the periodical within the volume

February–March

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

31-34

UT code for WoS article

000425747400006

EID of the result in the Scopus database

2-s2.0-85039698991