HU protein is involved in intracellular growth and full virulence of Francisella tularensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889506" target="_blank" >RIV/60162694:G44__/18:43889506 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/18:10393279
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1441588" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1441588</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/21505594.2018.1441588" target="_blank" >10.1080/21505594.2018.1441588</a>
Alternative languages
Result language
angličtina
Original language name
HU protein is involved in intracellular growth and full virulence of Francisella tularensis
Original language description
The nucleoid-associated HU proteins are small abundant DNA-binding proteins in bacterial cell which play an important role in the initiation of DNA replication, cell division, SOS response, control of gene expression and recombination. HU proteins bind to double stranded DNA non-specifically, but they exhibit high affinity to abnormal DNA structures as four-way junctions, gaps or nicks, which are generated during DNA damage. In many pathogens HU proteins regulate expression of genes involved in metabolism and virulence. Here, we show that the Francisella tularensis subsp. holarctica gene locus FTS_0886 codes for functional HU protein which is essential for full Francisella virulence and its resistance to oxidative stress. Further, our results demonstrate that the recombinant FtHU protein binds to double stranded DNA and protects it against free hydroxyl radicals generated via Fenton's reaction. Eventually, using an iTRAQ approach we identified proteins levels of which are affected by the deletion of hupB, among them for example Francisella pathogenicity island (FPI) proteins. The pleiotropic role of HU protein classifies it as a potential target for the development of therapeutics against tularemia.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Virulence
ISSN
2150-5594
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
754-770
UT code for WoS article
000430871000002
EID of the result in the Scopus database
2-s2.0-85053349268