Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F24%3A00560785" target="_blank" >RIV/60162694:G44__/24:00560785 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/23:10479151
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1330109/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1330109/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2023.1330109" target="_blank" >10.3389/fmicb.2023.1330109</a>
Alternative languages
Result language
angličtina
Original language name
Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensis
Original language description
The nucleoid-associated protein HU is a common bacterial transcription factor, whose role in pathogenesis and virulence has been described in many bacteria. Our recent studies showed that the HU protein is an indispensable virulence factor in the human pathogenic bacterium Francisella tularensis, a causative agent of tularemia disease, and that this protein can be a key target in tularemia treatment or vaccine development. Here, we show that Francisella HU protein is inhibited by Gp46, a protein of Bacillus subtilis bacteriophage SPO1. We predicted that Gp46 could occupy the F. tularensis HU protein DNA binding site, and subsequently confirmed the ability of Gp46 to abolish the DNA-binding capacity of HU protein. Next, we showed that the growth of Francisella wild-type strain expressing Gp46 in trans corresponded to that of a deletion mutant strain lacking the HU protein. Similarly, the efficiency of intracellular proliferation in mouse macrophages resembled that of the deletion mutant strain, but not that of the wild-type strain. These results, in combination with findings from a recent study on Gp46, enabled us to confirm that Gp46 could be a universal inhibitor of HU proteins among bacterial species.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
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Volume of the periodical
14
Issue of the periodical within the volume
December
Country of publishing house
CH - SWITZERLAND
Number of pages
10
Pages from-to
1330109
UT code for WoS article
001128729200001
EID of the result in the Scopus database
2-s2.0-85180525130