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Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F24%3A00560785" target="_blank" >RIV/60162694:G44__/24:00560785 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11160/23:10479151

  • Result on the web

    <a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1330109/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1330109/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fmicb.2023.1330109" target="_blank" >10.3389/fmicb.2023.1330109</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensis

  • Original language description

    The nucleoid-associated protein HU is a common bacterial transcription factor, whose role in pathogenesis and virulence has been described in many bacteria. Our recent studies showed that the HU protein is an indispensable virulence factor in the human pathogenic bacterium Francisella tularensis, a causative agent of tularemia disease, and that this protein can be a key target in tularemia treatment or vaccine development. Here, we show that Francisella HU protein is inhibited by Gp46, a protein of Bacillus subtilis bacteriophage SPO1. We predicted that Gp46 could occupy the F. tularensis HU protein DNA binding site, and subsequently confirmed the ability of Gp46 to abolish the DNA-binding capacity of HU protein. Next, we showed that the growth of Francisella wild-type strain expressing Gp46 in trans corresponded to that of a deletion mutant strain lacking the HU protein. Similarly, the efficiency of intracellular proliferation in mouse macrophages resembled that of the deletion mutant strain, but not that of the wild-type strain. These results, in combination with findings from a recent study on Gp46, enabled us to confirm that Gp46 could be a universal inhibitor of HU proteins among bacterial species.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Microbiology

  • ISSN

    1664-302X

  • e-ISSN

  • Volume of the periodical

    14

  • Issue of the periodical within the volume

    December

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    10

  • Pages from-to

    1330109

  • UT code for WoS article

    001128729200001

  • EID of the result in the Scopus database

    2-s2.0-85180525130