Fast and simple glucose assay based on filter paper as enzymes carrier using phone camera detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889568" target="_blank" >RIV/60162694:G44__/18:43889568 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/18:PU136070

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11696-018-0527-2" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-018-0527-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-018-0527-2" target="_blank" >10.1007/s11696-018-0527-2</a>

Result language

angličtina

Original language name

Fast and simple glucose assay based on filter paper as enzymes carrier using phone camera detection

Original language description

Glucose is a typical diagnostic marker of diabetes mellitus but it may be also helpful during diagnosis of some types of poisoning, immunological disorder or long-term stress at hospitalized patients. Diagnosis of diabetes mellitus in time is indispensable for prevention of life-threatening complication and so, accurate and easy device for non-invasive and accurate self-diagnosis is necessary. Simple assay based on filter paper with chitosan layer and with immobilized enzymes glucose oxidase and peroxidase and phone camera detection has been proposed for home-care glucose detection in urine. Data were processed in RGB (red-green-blue) color model when blue color channel showed the best sensitivity to changing glucose concentrations. Assay exerted linear regression from 0 to 4 mmol/l and limit of detection was set to be 166 A mu mol/l. Assay was not influenced by any interfering substances including maltose, sucrose, sorbitol or fructose or by urine matrix substance such as bovine serum albumin, ascorbic acid, uric acid or urea. Measurement of glucose from spiked urine samples reveals also no influence of the matrix on the assay. Long-term stability was measured and filter papers coated by enzymes showed excellent stability for at least 9 weeks without any special treatment or package. In comparison with differently modified filter paper, filter paper with both chitosan and enzymes showed the best sensitivity to changing glucose concentration. In a conclusion, simple (phone camera detection) and fast (3-min reaction) assay for quantitative detection of glucose from urine was optimized.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30107 - Medicinal chemistry

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers

ISSN

0366-6352

e-ISSN

—

Volume of the periodical

72

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

2719-2728

UT code for WoS article

000441699600004

EID of the result in the Scopus database

2-s2.0-85051661467