Targeted mass spectrometry analysis of Clostridium perfringens toxins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00537016" target="_blank" >RIV/60162694:G44__/19:00537016 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G33__/19:N0000006 RIV/62690094:18470/19:50015557
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468457/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468457/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/toxins11030177" target="_blank" >10.3390/toxins11030177</a>

Result language
angličtina
Original language name
Targeted mass spectrometry analysis of Clostridium perfringens toxins
Original language description
Targeted proteomics recently proved to be a technique for the detection and absolute quantification of proteins not easily accessible to classical bottom-up approaches. Due to this, it has been considered as a high fidelity tool to detect potential warfare agents in wide spread kinds of biological and environmental matrices. Clostridium perfringens toxins are considered to be potential biological weapons, especially the epsilon toxin which belongs to a group of the most powerful bacterial toxins. Here, the development of a target mass spectrometry method for the detection of C. perfringens protein toxins (alpha, beta, beta2, epsilon, iota) is described. A high-resolution mass spectrometer with a quadrupole-Orbitrap system operating in target acquisition mode (parallel reaction monitoring) was utilized. Because of the lack of commercial protein toxin standards recombinant toxins were prepared within Escherichia coli. The analysis was performed using proteotypic peptides as the target compounds together with their isotopically labeled synthetic analogues as internal standards. Calibration curves were calculated for each peptide in concentrations ranging from 0.635 to 1101 fmol/mu L. Limits of detection and quantification were determined for each peptide in blank matrices.
Czech name
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Czech description
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Type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology

Project
<a href="/en/project/VH20172020012" target="_blank" >VH20172020012: Preparation of the collection of biologically significant toxins with the support of European biological European biodefence laboratory network</a>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP) S - Specificky vyzkum na vysokych skolach I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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