A comparative analysis of protein virulence factors released via extracellular vesicles in two Candida albicans strains cultivated in a nutrient-limited medium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00537017" target="_blank" >RIV/60162694:G44__/19:00537017 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/19:00518294 RIV/00216208:11160/19:10400423

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0882401019302992" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0882401019302992</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micpath.2019.103666" target="_blank" >10.1016/j.micpath.2019.103666</a>

Result language

angličtina

Original language name

A comparative analysis of protein virulence factors released via extracellular vesicles in two Candida albicans strains cultivated in a nutrient-limited medium

Original language description

One of the pathways for the delivery of virulence effector molecules into the extracellular environment of Candida albicans relies on the release of membrane-bound carriers which are called extracellular vesicles (EVs). Only a few studies aimed at investigating Candida albicans extracellular vesicles protein cargo and its potential contribution to the pathogenesis of C. albicans infections have been conducted to date. In this study, we mainly focused on a search for proteins with a demonstrated linkage to pathogenesis in EVs isolated from two C. albicans strains, the model strain ATCC 90028 and the clinical isolate from a woman suffering from vulvovaginal candidiasis. For the purpose of mimicking one of many hostile conditions during a host-pathogen interaction, C. albicans strains in a nutrient-limited medium were cultivated. We have hypothesized that this unfavourable, stressful condition could contribute to the induction of virulence effector molecules being released at a more extensive rate. In conclusion, 34 proteins with an undisputed linkage to C. albicans pathogenesis were detected in the extracellular vesicle cargoes of both strains. In case of the clinical isolate strain, no unique virulence-associated proteins were detected. In the C. albicans ATCC 90028 model strain, three unique proteins were detected, namely: agglutinin-like protein 3 (Als3), secreted aspartic protease 8 (Sap8) and cell surface superoxide dismutase [Cu-Zn] 6 (Sod6).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microbial Pathogenesis

ISSN

0882-4010

e-ISSN

—

Volume of the periodical

136

Issue of the periodical within the volume

Nov

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

UNSP 103666

UT code for WoS article

000498333600011

EID of the result in the Scopus database

2-s2.0-85070760687