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The evaluation of oxidative damage of DNA after poisoning with nerve agents

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00555568" target="_blank" >RIV/60162694:G44__/19:00555568 - isvavai.cz</a>

  • Result on the web

    <a href="https://jab.zsf.jcu.cz/pdfs/jab/2019/04/04.pdf" target="_blank" >https://jab.zsf.jcu.cz/pdfs/jab/2019/04/04.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.32725/jab.2019.017" target="_blank" >10.32725/jab.2019.017</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The evaluation of oxidative damage of DNA after poisoning with nerve agents

  • Original language description

    The potency of three nerve agents (sarin, soman, tabun) to induce oxidative damage of DNA in lymphocytes, liver and brain during lethal or sublethal poisoning was investigated. The single strand breaks or oxidative base DNA damage was evaluated with the help of Comet assay and a specific enzyme able to detect oxidative bases of DNA (endonudease III). While sarin and soman administered at sublethal doses corresponding to 50% of their LD50 values were not able to induce oxidative damage of DNA, their lethal dose (LD50) ) induced the significant increase of the number of oxidative bases in DNA of hepatocytes. In addition, tabun administered at lethal dose (LD50) induced significant increase of the number of single strand breaks and oxidative bases of DNA in glial cells isolated from pontomedullar brain region. Thus, some nerve agents were able to induce oxidative damage in the peripheral as well as central compartment but only in the case of severe poisoning caused by lethal doses of nerve agents. This non-cholinergic effect of nerve agents has probably consequences with nerve agents-induced hypoxic status during acute cholinergic crisis and it can contribute to their long-term toxic effects.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Applied Biomedicine

  • ISSN

    1214-021X

  • e-ISSN

    1214-0287

  • Volume of the periodical

    17

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    6

  • Pages from-to

    225-230

  • UT code for WoS article

    000510179600004

  • EID of the result in the Scopus database

    2-s2.0-85078838902