Epigallocatechin gallate inhibits Francisella tularensis growth and suppresses the function of DNA-binding protein HU
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F24%3A00558857" target="_blank" >RIV/60162694:G44__/24:00558857 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0882401023000323?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0882401023000323?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.micpath.2023.105999" target="_blank" >10.1016/j.micpath.2023.105999</a>
Alternative languages
Result language
angličtina
Original language name
Epigallocatechin gallate inhibits Francisella tularensis growth and suppresses the function of DNA-binding protein HU
Original language description
Francisella tularensis is a highly infectious intracellular bacterium causing tularemia disease and is regarded as a potential biological weapon. The development of a vaccine, effective treatment, or prophylactic substances targeted against tularemia is in the forefront of interest and could help to prevent or mitigate possible malevolent acts by bioterrorism utilizing F. tularensis. The viability of F. tularensis, and thus of a tularemia disease outbreak, might potentially be suppressed by simple commonly available natural substances. Epigallocatechin gallate (EGCG) is contained in green tea and its antimicrobial effect has been described. Here, we show that EGCG can suppress F. tularensis growth and is able to reduce the bacterium's ability to replicate inside mouse bone marrow-derived macrophages (BMMs) without side effects on BMMs' own viability. We suggest one (but not the only) mechanism of EGCG action. We demonstrate that EGCG can block the main functions of HU protein, the important regulator of F. tularensis virulence, leading to overall attenuation of F. tularensis viability. EGCG can delay death of mice infected by F. tularensis and can be used as a prophylactic agent against tularemia disease. Postponing death by up to 2 days can provide sufficient opportunity to administer another treatment agent.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial Pathogenesis
ISSN
0882-4010
e-ISSN
1096-1208
Volume of the periodical
176
Issue of the periodical within the volume
Mar
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
105999
UT code for WoS article
000926792200001
EID of the result in the Scopus database
2-s2.0-85147802723