Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F25%3A00563135" target="_blank" >RIV/60162694:G44__/25:00563135 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1355872/full" target="_blank" >https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1355872/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2024.1355872" target="_blank" >10.3389/fmicb.2024.1355872</a>

Result language

angličtina

Original language name

Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis

Original language description

Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria and suppresses their intracellular replication. On the other hand, the subsequent infection with Francisella is able to revert to some extent the strong pro-inflammatory effect induced by OMVs in macrophages. Being derived from the bacterial surface, isolated OMVs may be considered a "non-viable mixture of Francisella antigens" and as such, they present a promising protective material. Immunization of mice with OMVs isolated from a virulent F. tularensis subsp. holarctica strain FSC200 prolonged the survival time but did not fully protect against the infection with a lethal dose of the parent strain. However, the sera of the immunized animals revealed unambiguous cytokine and antibody responses and proved to recognize a set of well-known Francisella immunoreactive proteins. For these reasons, Francisella OMVs present an interesting material for future protective studies.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-04010S" target="_blank" >GA17-04010S: Study of the secretion of outer membrane vesicles in Francisella tularensis and their role in the interaction with the host</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

1664-302X

Volume of the periodical

15

Issue of the periodical within the volume

Mar

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

1355872

UT code for WoS article

001190371700001

EID of the result in the Scopus database

2-s2.0-85188543234