Identification of Pairs of Organophosphorus Warfare Agents through Cholinesterase Reaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG45__%2F11%3A00445726" target="_blank" >RIV/60162694:G45__/11:00445726 - isvavai.cz</a>
Result on the web
<a href="http://vavtest.unob.cz/registr" target="_blank" >http://vavtest.unob.cz/registr</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Identification of Pairs of Organophosphorus Warfare Agents through Cholinesterase Reaction
Original language description
Construction of Detehit (nerve-agent detector), a colorimetric biosensor of nerve agents, enables its utilization for selective analysis of nerve agents based on the different ability of nucleophilic reagents to reactivate enzyme-inhibitor complexes in the phase before dealkylation. For this purpose mono- and bispyridinium aldoximes, common in treatment of nerve agent poisoning, are used. For a positive identification of a pair of organophosphates, a neural network analysis was used. The analysis was processed based on spectral data of the intensity of color changes of the surface of a cotton cloth reaction zone with immobilized and stabilized enzyme acetylcholinesterase. Color changes are based on Ellman's reaction. Intensity of these changes dependson activity of the enzyme after inhibition and reactivation of enzyme-inhibitor complex. The following nerve agents were identified: sarin, soman, tabun, cyclosarin, VX, and R-33.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
KA - Militarism
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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