Determination of patulin and hydroxymethylfurfural in beverages by UPLC-PDA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F21%3AN0000029" target="_blank" >RIV/60193697:_____/21:N0000029 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/21:43919549 RIV/00216208:11310/21:10435867 RIV/00216305:26310/20:PU144083
Result on the web
<a href="https://www.wageningenacademic.com/doi/abs/10.3920/WMJ2020.2587" target="_blank" >https://www.wageningenacademic.com/doi/abs/10.3920/WMJ2020.2587</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3920/WMJ2020.2587" target="_blank" >10.3920/WMJ2020.2587</a>
Alternative languages
Result language
angličtina
Original language name
Determination of patulin and hydroxymethylfurfural in beverages by UPLC-PDA
Original language description
The present study describes using molecularly imprinted polymer (MIP) technology for determination of patulin (PAT) and 5-hydroxymethylfurfural (5-HMF) in beverages by ultra-high performance liquid chromatography coupled to photodiode array (UPLC-PDA). PAT (4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one) is a mycotoxin produced by Penicillium fungi and Penicillium expansum is probably the most commonly encountered species that infects apples during their growth, harvest, storage or processing. The occurrence of PAT as a natural contaminant of apples is a worldwide problem. 5-HMF (also known as 5-(hydroxymethyl) furan-2-carbaldehyde), is formed in the Maillard reaction as well as during caramelisation. It is a good storage time-temperature marker and flavour indicator, especially in beverages such as wine, beer, but also cider and apple juice which may contain PAT. PAT and 5-HMF were separated within 2 min using a Luna Omega C18 column and the PDA detector wavelength was set to 276 nm. The validation parameters of the analytical method such as linearity, limit of detection, limit of quantification, accuracy and precision were tested. The calibration curves were linear at least in the range 50-1000 ng/ml with a good linearity (R2>0.999) for both analytes, the limit of detection and the limit of quantification for PAT and 5-HMF were in the range 4.9-6.6 and 16.1-21.8 μg/l, respectively. The recoveries of the selected analyte were in the range 61.9-109.0% with a precision of <8.2% (relative standard deviation (RSD)) for PAT and in the range 50.8-98.0% with a precision of <10.0% (RSD) for 5-HMF. The validated procedure was successfully applied for the analysis of PAT and 5-HMF in beverages from retail shops.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
World Mycotoxin Journal
ISSN
1875-0710
e-ISSN
1875-0796
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
41-48
UT code for WoS article
000618184100004
EID of the result in the Scopus database
2-s2.0-85101930491