Ergot Alkaloids in Rye Flour Marketed in Czech Republic: Comparison Between ELISA and LC–MS Methodologies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F24%3AN0000007" target="_blank" >RIV/60193697:_____/24:N0000007 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/24:43928942

Result on the web

<a href="https://link.springer.com/article/10.1007/s12161-024-02612-x" target="_blank" >https://link.springer.com/article/10.1007/s12161-024-02612-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-024-02612-x" target="_blank" >10.1007/s12161-024-02612-x</a>

Result language

angličtina

Original language name

Ergot Alkaloids in Rye Flour Marketed in Czech Republic: Comparison Between ELISA and LC–MS Methodologies

Original language description

Ergot alkaloids (EAs) are toxins produced by Claviceps purpurea fungi that may infect cereals, particularly rye. The present study describes the comparison between ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) and ELISA for the determination of twelve ergot alkaloids in rye flour. The sample preparation for LC–MS analysis was done by the QuEChERS method and clean-up via freeze-out. The analytical LC–MS method was fully validated, and the validation parameters such as linearity, limit of quantification (LOQ), precision, accuracy and matrix effect were tested. The calibration curves were linear (R2 > 0.99) for all ergot alkaloids. The recoveries ranged from 74 to 104%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 17% for any analytes. The analytical LC–MS method and ELISA were applied to 27 samples of rye flour commercially available from retail shops in the Czech Republic. A positive correlation was found between the samples measured by ELISA and LC–MS. Ergot alkaloid concentra- tions measured by these methods were compared with the European legislation resulting in 5 samples exceeding the set limit of 500 µg/kg with 2 samples exceeding this limit 10 and 20 times, respectively. With the new legislative limit coming into effect in July 2024, more than half of the samples would not comply with the new established limit of 250 µg/kg.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Analytical Methods

ISSN

1936-9751

e-ISSN

1936-976X

Volume of the periodical

17

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

787-794

UT code for WoS article

001185625000001

EID of the result in the Scopus database

2-s2.0-85187948595