The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41110%2F20%3A83945" target="_blank" >RIV/60460709:41110/20:83945 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/20:83945
Result on the web
<a href="https://doi.org/10.17221/127/2020-CJAS" target="_blank" >https://doi.org/10.17221/127/2020-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/127/2020-CJAS" target="_blank" >10.17221/127/2020-CJAS</a>
Alternative languages
Result language
čeština
Original language name
The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
Original language description
The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30. Spermatozoa of good freezers are represented more frequently in the fast and me
Czech name
The structure of subpopulations of stallion spermatozoa after thawing differs between good and poor freezers
Czech description
The aim of this study was to evaluate differences in the presence of sperm subpopulations in frozen-thawed semen in stallions with different freezability. The motility of individual spermatozoa of 24 stallions from 15 breeds was evaluated using computer-assisted sperm analysis (CASA) immediately after thawing (T0) and after 30 min of incubation (T30). In accordance with our previous studies, samples were initially divided based on their total motility into categories of good (GF) and poor (PF) freezers. K-means cluster analysis of kinematic parameters of spermatozoa was used to divide motile sperm into three subpopulations. Analysis of variance was used to evaluate differences in the subpopulations between GF and PF stallions at the times of incubation T0 and T30. Statistically significant differences were found in most kinematic parameters between PF and GF stallions as well as between the times of incubation T0 and T30. Spermatozoa of good freezers are represented more frequently in the fast and me
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
1805-9309
Volume of the periodical
65
Issue of the periodical within the volume
11
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
403-410
UT code for WoS article
000595926000001
EID of the result in the Scopus database
2-s2.0-85097493800