Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76958" target="_blank" >RIV/60460709:41210/18:76958 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2478/sab-2018-0026" target="_blank" >http://dx.doi.org/10.2478/sab-2018-0026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/sab-2018-0026" target="_blank" >10.2478/sab-2018-0026</a>
Alternative languages
Result language
angličtina
Original language name
Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa
Original language description
The objective of our study was to determine the effect of 5 percent of CO2 atmosphere and time of sample dilution on results of in vitro analysis of stallion semen. Frozen-thawed semen from 14 stallions was incubated either in incubator or in a water bath, diluted prior to analysis or immediatelly after thawing. The following qualitative parameters were assessed after thawing T0 and after 30 min T3 motility in 3 sperm subpopulations slow, medium, fast defined by cluster analysis of parameters obtained by Computer Assisted Sperm Analysis, viability, acrosome and mitochondrial integrity. The slow subpopulation was only significantly reduced in diluted samples in CO2 atmosphere at T0 P lower than 0,05. In diluted samples the incubation time sig-nificantly affected distribution of fast, slow, and medium subpopulations in CO2 and CO2 free atmosphere P lower than 0,05, respec-tively. Viability, acrosome and mitochondrial integrity were not affected by CO
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientia Agriculturae Bohemica
ISSN
1211-3174
e-ISSN
1805-9430
Volume of the periodical
49
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
201-208
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85054361235