Detection of Resistance Genes in Solanaceae Using DNA Markers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F01%3A00002739" target="_blank" >RIV/60460709:41210/01:00002739 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection of Resistance Genes in Solanaceae Using DNA Markers
Original language description
As model botanic species were selected tomato (Lycopersicon esculentum) and potato (Solanum tuberosum ssp. tuberosum). The potato was assessed for the collection of tetraploidic gene sources. The tomato was assessed for gene sources - varieties of the F1generation type and the segregating F2 generation. The potato's resistance against Ro1 of the Globodera rostochiensis pathotype was marked with a PCR amplification of the Gro 1.2.3 loci in chromosome II.. To assess the resistance, the results of experiments conducted by Leister et al. (1997) were used. One loci of quantitative resistant - PCR marker has a close gene link with the monitored resistance. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. The experiments were based on tests by Williamson et al. (1994). Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR
Czech name
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Czech description
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Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
47
Publisher name
Ústav molekulární biologie rostlin AV ČR
Place of publication
České Budějovice
Event location
Třeboň
Event date
Jan 1, 2001
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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