DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005226" target="_blank" >RIV/60460709:41210/03:00005226 - isvavai.cz</a>
Alternative codes found
RIV/60460709:_____/03:00005226
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DETECTION OF HOP STUNT VIROID WITHIN POLLEN GRAINS OF INFECTED PEACH TREES: EVIDENCE USING RT-PCR AND DOT BLOT HYBRIDIZATION
Original language description
Hop stunt viroid (HSVd) is the viroid with the broadest host range known so far, also including stone fruit trees. Until now, six viroids have been shown to be transmitted through seeds to seedlings, in one or more hosts, four of them have been detectedin pollen, cucumber strain of HSVd being among them. High incidence of HSVd infections of peach trees was recently found in the Czech Republic indicating the possibility of spreading via pollen. That is why we tested the presence of HSVd in pollen grains. Closed flowers from peach trees of cultivars Sunhaven and NJC 103 known to be infected by HSVd were collected in March. Pollen grains were separated from air dried anthers and total RNA was extracted from 10 - 20 mg of pollen or intact anthers and from100 mg of leaves or petals. PCR and molecular hybridization methods were used for HSVd detection. HSVd was consistently detected in pollen, anthers, petals as well as in leaves in each of at least three repetitions. This preliminary resul
Czech name
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Czech description
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Project
<a href="/en/project/QC1359" target="_blank" >QC1359: Development of complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for developing of fruit trees certification system in the Czech Republic.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Book of abstract 19th Int. Symp. Virus and Virus - like Diseases of Temperature Fruit Crops
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
117
Publisher name
ISHS
Place of publication
Valencia
Event location
Valencia
Event date
Jul 21, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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