Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A%23%23%2312265" target="_blank" >RIV/60460709:41210/05:###12265 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.
Original language description
High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detect
Czech name
Vývoj metody RT-PCR pro detekci viroidu latentní mozaiky broskvoně a viroidu zakrslosti chmele pro účely certifikace sadbového materiálu
Czech description
High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detect
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QC1359" target="_blank" >QC1359: Development of complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for developing of fruit trees certification system in the Czech Republic.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Horticulturae
ISSN
0567-7572
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
657
Country of publishing house
BE - BELGIUM
Number of pages
5
Pages from-to
391-395
UT code for WoS article
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EID of the result in the Scopus database
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