DEVELOPMENT OF PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F03%3A00005230" target="_blank" >RIV/60460709:41210/03:00005230 - isvavai.cz</a>
Alternative codes found
RIV/60460709:_____/03:00005230
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DEVELOPMENT OF PCR METHOD OF PEACH LATENT MOSAIC VIROID AND HOP STUNT VIROID DETECTION FOR CERTIFICATION OF PLANTING MATERIAL
Original language description
High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usin gany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be dete
Czech name
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Czech description
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Project
<a href="/en/project/QC1359" target="_blank" >QC1359: Development of complex procedures of diagnostics of economically important and quarantine phytopatogenic organisms for developing of fruit trees certification system in the Czech Republic.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Book of abstract 19th Int. Symp. Virus and Virus - like Diseases of Temperature Fruit Crops
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
115
Publisher name
ISHS
Place of publication
Valencia
Event location
Valencia
Event date
Jul 21, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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