Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7180" target="_blank" >RIV/60460709:41210/04:7180 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of proteasome inhibitor MG132 on in vitro maturation of porcine oocytes

Original language description

The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes wereblocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from me

Czech name

Účinek inhibitoru proteazómu MG132 na meiotické zrání prasečích oocytů

Czech description

The study aimed to demonstrate the dependence of meiotic maturation in pig oocytes on the activity of the protease complex proteasome. The proteasome inhibitor MG132blocked the exit of maturing pig oocytes from I metaphase stage. 75% of the oocytes wereblocked at MI stage when they were cultured with 10 M MG132. The blocking effect of MG132 was expressed only when the oocytes were exposed an inhibitor before the 18th hour of culture. The effects of MG132 are fully reversible. However a significant proportion of oocytes (46%) cultured for 48 hours in MG132 supplemented medium and then for 24 h in MG132-free medium did not block meiosis at the stage of metaphase II and underwent spontaneous parthenogenetic activation. On the basis of our data we can conclude that exit from the metaphase I stage of meiosis is preoteasome-dependent in pig oocytes matured in vitro. On the other hand, our data also indicate that other proteasome-independent events are involved in regulating the exit from me

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zygote

ISSN

0967-1994

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

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Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

157-162

UT code for WoS article

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EID of the result in the Scopus database

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