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Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001104" target="_blank" >RIV/00027014:_____/09:#0001104 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

  • Original language description

    In fully grown pig oocytes, meiotic maturation in vitro is slowed by inhibition of histone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, the culture with TSA has no significant effect on the meitic maturation.Growing oocytes treated with TSA mature mainly to the stage of metaphase I. The ratio of oocytes maturing to the stage of metaphase II is very limited. After the transient exposure to TSA, the maturation of growing oocytes with partial meiotic competencetakes another course. When these oocytes are first cultured in TSA-free medium, then cultured for another 24 hours with 100 nM TSA and finally they are again cultured in TSA-free medium for 24 hours, the ratio of oocytes maturing to the stage of metaphase II is significantly increased and it reached 59%. When oocytes are cultured for the same time without transient exposure to TSA, only 19% matured to the stage of metaphase II.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/QG50052" target="_blank" >QG50052: Reproductive biotechniques in pigs</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Zygote

  • ISSN

    0967-1994

  • e-ISSN

  • Volume of the periodical

    17

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

  • UT code for WoS article

    000271184000004

  • EID of the result in the Scopus database