Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7852" target="_blank" >RIV/60460709:41210/04:7852 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku

Original language description

Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control, and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene codinga yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, thus enabling to followthe heavy metal tolerance and uptake changes as function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. Determination of Zn content in above ground biomass did not confirm higher uptake by transgenic plants. The Ni content was significantly increased in above ground biomass of HisGUS construct. GUS constru

Czech name

The evaluation of Cd, Zn and Ni accumulation ability of transgenic tobacco bearing different transgenes

Czech description

Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control, and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene codinga yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, thus enabling to followthe heavy metal tolerance and uptake changes as function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. Determination of Zn content in above ground biomass did not confirm higher uptake by transgenic plants. The Ni content was significantly increased in above ground biomass of HisGUS construct. GUS constru

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA526%2F02%2F0293" target="_blank" >GA526/02/0293: The role of mycorrhizal fungi in uptake of heavy metals by transgenic plants: a potential phytoremediation model</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant, Soil and Environment

ISSN

1214-1178

e-ISSN

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Volume of the periodical

50

Issue of the periodical within the volume

12

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

513-517

UT code for WoS article

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EID of the result in the Scopus database

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