Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7847" target="_blank" >RIV/60460709:41210/04:7847 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Schopnost transgenního tabáku připraveného kombinací genu pro metallothionein a genu pro histidinovou kotvu akumulovat a tolerovat kadmium

Original language description

Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and a genetically modified line of the same variety, bearing the transgene coding for the polyhistidine cluster, combined with yeast metallothionein (HisCUP) was tested for cadmium accumulation and tolerance. The screening test was conducted on sand nutrient medium with addition of Knop?s nutrient solution modified by addition of various cadmium doses. Significantly increasing Cd accumulation in above ground biomass of HisCUP tobaccogrowing in Cd concentration 0.2 and 0.6 mg Cd.l-1 of nutrient solution was determined comparing to the control. A dose of 5.4 mg Cd.l-1 induced cadmium toxicity symptoms of control tobacco. The highest dose of Cd was lethal for this treatment. HisCUP showed increasing plant resistance against stress response induced by cadmium. The experiment was continued in model pot experiments with two various polluted soils. The Cd content in the above ground biomass of transgenic tobacco was incre

Czech name

Cadmium tolerance and accumulation in transgenic tobacco plants with a yeast metallothionein combined with a polyhistidine tail

Czech description

Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and a genetically modified line of the same variety, bearing the transgene coding for the polyhistidine cluster, combined with yeast metallothionein (HisCUP) was tested for cadmium accumulation and tolerance. The screening test was conducted on sand nutrient medium with addition of Knop?s nutrient solution modified by addition of various cadmium doses. Significantly increasing Cd accumulation in above ground biomass of HisCUP tobaccogrowing in Cd concentration 0.2 and 0.6 mg Cd.l-1 of nutrient solution was determined comparing to the control. A dose of 5.4 mg Cd.l-1 induced cadmium toxicity symptoms of control tobacco. The highest dose of Cd was lethal for this treatment. HisCUP showed increasing plant resistance against stress response induced by cadmium. The experiment was continued in model pot experiments with two various polluted soils. The Cd content in the above ground biomass of transgenic tobacco was incre

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA526%2F02%2F0293" target="_blank" >GA526/02/0293: The role of mycorrhizal fungi in uptake of heavy metals by transgenic plants: a potential phytoremediation model</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Biodeterioration & Biodegradation

ISSN

0964-8305

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

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Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

233-237

UT code for WoS article

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EID of the result in the Scopus database

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