Effect of extraction methods on the chemical composition and antimicrobial activity of Nigella sativa seed essential oils

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A9934" target="_blank" >RIV/60460709:41210/04:9934 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41610/04:9934

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Vliv použité extrakční metody na chemické složení a antimikrobiální aktivitu silice ze semen Nigella sativa

Original language description

Nigella sativa Linn. (Ranunculaceae) is used in folk medicine all over the world for the treatment of a number of diseases. Its seed essential oil (EO) has previously demonstrated a wide range of biological activities, including antimicrobial and antifungal effects. In our study, we aimed to determine the influence of three different extraction methods on the chemical composition and antimicrobial activity of N. sativa seed EOs. EOs were extracted by hydrodistillation (H) of seeds and by steam distillation (SD) of crude oils obtained by solvent extraction (SE) or supercritical fluid extraction (SFE) and then analysed by gas chromatography-mass spectrometry. Antimicrobial activities were expressed as minimum inhibitory concentration (MIC) values using the broth microdilution method. The results showed that EOs tested differ markedly in their chemical compositions and antimicrobial activities. EO obtained by H was dominated by p-cymene (46.2%), whereas the major constituent identified in

Czech name

Vliv použité extrakční metody na chemické složení a antimikrobiální aktivitu silice ze semen Nigella sativa

Czech description

Nigella sativa Linn. (Ranunculaceae) is used in folk medicine all over the world for the treatment of a number of diseases. Its seed essential oil (EO) has previously demonstrated a wide range of biological activities, including antimicrobial and antifungal effects. In our study, we aimed to determine the influence of three different extraction methods on the chemical composition and antimicrobial activity of N. sativa seed EOs. EOs were extracted by hydrodistillation (H) of seeds and by steam distillation (SD) of crude oils obtained by solvent extraction (SE) or supercritical fluid extraction (SFE) and then analysed by gas chromatography-mass spectrometry. Antimicrobial activities were expressed as minimum inhibitory concentration (MIC) values using the broth microdilution method. The results showed that EOs tested differ markedly in their chemical compositions and antimicrobial activities. EO obtained by H was dominated by p-cymene (46.2%), whereas the major constituent identified in

Type

D - Article in proceedings

CEP classification

GM - Food industry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F02%2F0257" target="_blank" >GA525/02/0257: Screening of antibacterial and anti-inflammatory activity of selected species of genus Nigella L.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník abstraktů [CD-ROM]: 35th International symposium on essential oils

ISBN

nemá

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

0-0

Publisher name

ISEO

Place of publication

Messiana, Itálie

Event location

Messiana, Itálie

Event date

Sep 29, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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