Effect of L-canavanine on in vitro matuartion of pig oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11692" target="_blank" >RIV/60460709:41210/05:11692 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of L-canavanine on in vitro matuartion of pig oocytes

Original language description

In the present study we investigated the role of NO-dependent events in meiotic maturation in pig oocytes. It has been suggested that NO-NOS has important physiological roles in a variety of reproductive processes such as follicular development, ovulation, and spermatogenesis. The synthesis of NO is catalyzed by three distinct isoforms of NOS: neuronal (nNOS), endothelial NO (eNOS) and inducible NOS (iNOS). Of the three NOS isoenzymes, the ovaries express eNOS and iNOS but not nNOS. In the present study, we investigated the role of iNOS on meiotic maturation of pig oocytes in vitro. Cumulus-oocyte complexes (COC) collected from porcine ovaries were cultured in a modified M199 medium with iNOS inhibitors L-canavanine. The addition of L-canavanine to theculture medium had a significant impact on meiotic development, and L-canavanine markedly inhibited the oocyte meiotic maturation. This iNOS inhibitor increased the percentage of atypical oocytes compared with the control group.

Czech name

Vliv L-canavaninu na in vitro meiotické zrání prasečích oocytů

Czech description

In the present study we investigated the role of NO-dependent events in meiotic maturation in pig oocytes. It has been suggested that NO-NOS has important physiological roles in a variety of reproductive processes such as follicular development, ovulation, and spermatogenesis. The synthesis of NO is catalyzed by three distinct isoforms of NOS: neuronal (nNOS), endothelial NO (eNOS) and inducible NOS (iNOS). Of the three NOS isoenzymes, the ovaries express eNOS and iNOS but not nNOS. In the present study, we investigated the role of iNOS on meiotic maturation of pig oocytes in vitro. Cumulus-oocyte complexes (COC) collected from porcine ovaries were cultured in a modified M199 medium with iNOS inhibitors L-canavanine. The addition of L-canavanine to theculture medium had a significant impact on meiotic development, and L-canavanine markedly inhibited the oocyte meiotic maturation. This iNOS inhibitor increased the percentage of atypical oocytes compared with the control group.

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the International Scientific Conference 4th Biological Days: Progress in Biology

ISBN

80-8050-864-X

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

403-404

Publisher name

VSP

Place of publication

Nitra

Event location

Nitra

Event date

Sep 8, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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