Role of nitric oxide in meiotic resumption of pig oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11690" target="_blank" >RIV/60460709:41210/05:11690 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Role of nitric oxide in meiotic resumption of pig oocytes

Original language description

Nitric oxide (NO) is one of the free radicals that are implicated in a variety of intracellular signalling mechanisms. NO is synthesized from L-arginine by three different nitric oxide synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS)and inducible NOS (iNOS). It has been suggested that NO-NOS has important physiological roles in a variety of reproductive processes such as follicular development, ovulation, and spermatogenesis. In the present study we investigated the role of NO-dependent events in meiotic resumption in pig oocytes. The mechanisms that promote the resumption of meiosis in mammalian oocytes remain poorly understood. INOS specific inhibitor AG significantly blocked the meiotic resumption of the oocytes in a dose-dependent manner. The addition of L-NAME to the culture medium had no significant effect on meiotic resumption and GVBD. Our results suggest that the iNOS-derived NO pathway plays important roles in pig oocyte meiotic maturation, especially in

Czech name

Úloha oxidu dusnatého během meiotického znovuzahájení prasečích oocytů

Czech description

Nitric oxide (NO) is one of the free radicals that are implicated in a variety of intracellular signalling mechanisms. NO is synthesized from L-arginine by three different nitric oxide synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS)and inducible NOS (iNOS). It has been suggested that NO-NOS has important physiological roles in a variety of reproductive processes such as follicular development, ovulation, and spermatogenesis. In the present study we investigated the role of NO-dependent events in meiotic resumption in pig oocytes. The mechanisms that promote the resumption of meiosis in mammalian oocytes remain poorly understood. INOS specific inhibitor AG significantly blocked the meiotic resumption of the oocytes in a dose-dependent manner. The addition of L-NAME to the culture medium had no significant effect on meiotic resumption and GVBD. Our results suggest that the iNOS-derived NO pathway plays important roles in pig oocyte meiotic maturation, especially in

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Journal of Applied Biomedicine

ISBN

1214-021X

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

21-22

Publisher name

Biologická fakulta

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Oct 24, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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