Nitric oxide and meiotic competence of porcine oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F11%3A51054" target="_blank" >RIV/60460709:41210/11:51054 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/11:#0001458

Result on the web

<a href="http://dx.doi.org/10.1017/S1751731111000565" target="_blank" >http://dx.doi.org/10.1017/S1751731111000565</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1751731111000565" target="_blank" >10.1017/S1751731111000565</a>

Result language

angličtina

Original language name

Nitric oxide and meiotic competence of porcine oocytes

Original language description

Nitric oxide (NO), produced by NO-Synthase (NOS) enzyme can fulfil a regulatory role in growing period of porcine oocytes. The objective of this work was to ascertain the role of NO in the growth phase of pig oocytes and its influence on the acquisitionof meiotic competence. We demonstrated that the selective competitive iNOS inhibitor, aminoguanidine, and also the nonselective NOS inhibitor L-NAME, block meiotic maturation of oocytes. NOS inhibitors influence even competent oocytes in the first stageof meiotic metaphase. However, blockage is less effective than at the beginning of meiotic maturation. The number of parthenogenetically activated competent oocytes greatly increased in a pure medium after inhibitor reversion. A large quantity of NO externally added to the in vitro cultivation environment disrupts the viability of oocytes. The effectiveness of the inhibitor can be reversed in oocytes by an NO donor in a very low concentration.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GD523%2F08%2FH064" target="_blank" >GD523/08/H064: Biotechnologies of mammalian gametes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal

ISSN

1751-7311

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1398-1405

UT code for WoS article

000294412300009

EID of the result in the Scopus database

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