The Role of Nitric Oxide Synthase in Porcine Oocytes Meiotic Maturation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A7178" target="_blank" >RIV/60460709:41210/04:7178 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The Role of Nitric Oxide Synthase in Porcine Oocytes Meiotic Maturation

Original language description

Nitric oxide Synthase (NOS) has been considered as a highly regulated enzyme that can produce Nitric oxide, which is known as important signalling molecule. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation.In porcine oocytes were detected two NOS isoforms (from three known) NOS II and NOS III at all three studied stages (germinal vesicle, first and second meiotic metaphase). During the culture the amount of NO significantly increased with the stage of meiotic maturation. The addition of NOS inhibitor (L-NAME) significantly decreased the rate of oocytes at the second meiotic metaphase stage. Inactive NOS inhibitor conformer D-NAME did not affect the oocytes maturation. Our results indicate that NOS is involved in process of porcine oocyte meiotic maturation by producing NO.

Czech name

Úloha NO-syntázy na meiotické zrání prasečích oocytů

Czech description

Nitric oxide Synthase (NOS) has been considered as a highly regulated enzyme that can produce Nitric oxide, which is known as important signalling molecule. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation.In porcine oocytes were detected two NOS isoforms (from three known) NOS II and NOS III at all three studied stages (germinal vesicle, first and second meiotic metaphase). During the culture the amount of NO significantly increased with the stage of meiotic maturation. The addition of NOS inhibitor (L-NAME) significantly decreased the rate of oocytes at the second meiotic metaphase stage. Inactive NOS inhibitor conformer D-NAME did not affect the oocytes maturation. Our results indicate that NOS is involved in process of porcine oocyte meiotic maturation by producing NO.

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Výzkum v chovu skotu

ISBN

0139-7265

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

44-45

Publisher name

Výzkumný ústav pro chov skotu, s. r. o. Rapotín

Place of publication

Rapotín

Event location

Velké Losiny

Event date

Sep 16, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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