Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11536" target="_blank" >RIV/60460709:41210/05:11536 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nitric Oxide Synthase and Nitric Oxide Importance for Meiotic Maturation of Pig Oocytes

Original language description

Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabol

Czech name

Význam NO-syntázy a oxidu dusnatého pro meiotické zrání oocytu prasete

Czech description

Nitric oxide synthase (NOS) has been considered as a highly regulated enzyme that can produce nitric oxide (NO) from molecular oxygen and L-arginine. Three isoforms of NOS are known neuronal, inducible and endothelial. NO is an important signalling molecule that mediates many physiological processes. The aim of our study was to assess whether NOS is involved in porcine oocytes meiotic maturation by NO production. The expression of NOS was studied in three stages of porcine oocytes meiotic maturation, germinal vesicle (0 h), first meiotic metaphase (24 h) and second meiotic metaphase stage (48 h), using western blotting method. The activity of NOS was observed by measurement of NO metabolites, nitrites and nitrates, with Griess reagents. Two NOS isoforms were detected in porcine oocytes: inducible and endothelial nitric oxide synthase. These isoforms were detected in all three stages of porcine oocyte meiotic maturation. The results of NO measurement showed that the amount of NO metabol

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Journal of Applied Biomedicine

ISBN

1214-021X

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

8-8

Publisher name

Biologická fakulta

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Oct 26, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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