Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11703" target="_blank" >RIV/60460709:41210/05:11703 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)

Original language description

There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called ?ageing?, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.

Czech name

Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF

Czech description

There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called ?ageing?, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Volume of the periodical

50

Issue of the periodical within the volume

7

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

300-310

UT code for WoS article

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EID of the result in the Scopus database

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