The impact of some plant hormones on the metabolism of proline in wheat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A15049" target="_blank" >RIV/60460709:41210/06:15049 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The impact of some plant hormones on the metabolism of proline in wheat

Original language description

This paper reports the results of experiments in investigating the effect of 24-epibrassinolide (24-epi) and cytokinin (benzylaminopurine-BAP) on the activity of glutamate kinase (EC 2.7.2.11), chlorophyll and proline content in leaves of spring wheat. Proline plays an important role in plants in the regulation of their metabolism during stresses. The experiments were carried out in field and the preparations were applied in a spray in concentration 10-6 M. The enzyme glutamate kinase (GK) starts the biosynthesis of proline from glutamate and its activity was determined by the modified hydroxamate method1,2. Chlorophyll content was assessed spectrophotometrically from acetonic extract. The activity of GK grew up to 132.5 % (24-epi) and 181.5 % (BAP) incomparison with the control plants. Both preparations have stimulating effect on the chlorophyll content. Amino acid analysis of plant extracts was carried out by AccQ*Tag method3. HPLC system with a fluorescence detection was used in th

Czech name

Vliv některých rostlinných hormonů na metabolismus prolinu pšenice

Czech description

This paper reports the results of experiments in investigating the effect of 24-epibrassinolide (24-epi) and cytokinin (benzylaminopurine-BAP) on the activity of glutamate kinase (EC 2.7.2.11), chlorophyll and proline content in leaves of spring wheat. Proline plays an important role in plants in the regulation of their metabolism during stresses. The experiments were carried out in field and the preparations were applied in a spray in concentration 10-6 M. The enzyme glutamate kinase (GK) starts the biosynthesis of proline from glutamate and its activity was determined by the modified hydroxamate method1,2. Chlorophyll content was assessed spectrophotometrically from acetonic extract. The activity of GK grew up to 132.5 % (24-epi) and 181.5 % (BAP) incomparison with the control plants. Both preparations have stimulating effect on the chlorophyll content. Amino acid analysis of plant extracts was carried out by AccQ*Tag method3. HPLC system with a fluorescence detection was used in th

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings from XX. Biochemický zjazd held in Piešťany 12. - 16. September 2006

ISBN

80-969532-6-5

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

245-245

Publisher name

Slovenská spoločnosť pre biochémiu a molekulárnu biológiu

Place of publication

Piešťany

Event location

Piešťany

Event date

Sep 12, 2006

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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