Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A16318" target="_blank" >RIV/60460709:41210/06:16318 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/06:00044605

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enzymatic and protein differences between infective larvae of Trichostrongylus colubriformis conditioned or not conditioned to hypobiosis

Original language description

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio- Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200 ? 220 kDa) decreased in range 32.3 ? 35.4 % and the amount of sixpolypeptides (20 ? 28 kDa) increased in range 20.0 ? 27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysiswere observed between 21 and 34 kDa.

Czech name

Enzymatické a proteinové změny mezi infekčními larvami richostrongylus colubriformis indukovanými a neindukovanými k hypobióze

Czech description

The differences in protein and enzymatic profiles of infective larvae (L3) of Trichostrongylus colubriformis, both induced and non-induced to hypobiosis, have been evaluated by means of SDS-PAGE and densitometric analysis as well as by semiquantitative micromethod API-ZYM (Bio- Mérieux, France). Quantitative differences were identified in protein levels between the induced and non-induced larvae, where the amount of two polypeptides (200 ? 220 kDa) decreased in range 32.3 ? 35.4 % and the amount of sixpolypeptides (20 ? 28 kDa) increased in range 20.0 ? 27.0 % in the samples of induced larvae. In contrast to non-induced larvae, on gelatin-substrate gel in L3 in vitro released (IVR) proteases from larvae conditioned to hypobiosis, zones of proteolysiswere observed between 21 and 34 kDa.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Helmintologia

ISSN

0440-6605

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

2

Country of publishing house

SK - SLOVAKIA

Number of pages

5

Pages from-to

64-68

UT code for WoS article

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EID of the result in the Scopus database

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