First report of Peach latent mosaic viroid infecting peach in Egypt

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A26261" target="_blank" >RIV/60460709:41210/08:26261 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

First report of Peach latent mosaic viroid infecting peach in Egypt

Original language description

Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 ?l) of total nucleic acids, extracted from about 2 mg of leaftissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 ?l) also were used in RT-PCR as described by Loreti et al. (2). Only one of

Czech name

První nález viroidu latentní mozaiky broskvoně v Egyptě

Czech description

Peach latent mosaic viroid (PLMVd) is a widespread pathogen of stone fruit trees in some European and Mediterranean countries, and also in Northern America. To access the presence of the viroid in Egypt, a survey was conducted covering five commercial peach orchards in El Khatatba region in Al Minufiya governorate. During 2003-2004, 73 peach trees (cv. Florida grafted on Nemagard rootstock) were visually inspected and sampled. No symptoms characteristic of PLMVd infection as mosaic, delayed vegetation or fruit suture cracking were observed. All samples were tested for the presence of PLMVd using dot-blot hybridization and reverse transcription-polymerase chain reaction (RT-PCR). Aliquots (5 ?l) of total nucleic acids, extracted from about 2 mg of leaftissues, were spotted onto positively charged nylon membranes and hybridized under stringent conditions using a digoxigenin-labeled riboprobe (1). The extracts (1 ?l) also were used in RT-PCR as described by Loreti et al. (2). Only one of

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QG60123" target="_blank" >QG60123: Research and innovation of diagnostic procedures for economically significant, controlled and quarantine plant pathogenic organisms for the certification program of fruit trees with emphasis on molecular methods</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Disease

ISSN

0191-2917

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

92

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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