Identification and molecular characterisation of Peach latent mosaic viroid isolate infecting peach in Egypt

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F08%3A27646" target="_blank" >RIV/60460709:41210/08:27646 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification and molecular characterisation of Peach latent mosaic viroid isolate infecting peach in Egypt

Original language description

In Egypt, a survey covering five commercial peach orchards of the newreclaimed lands, El Khatatba region in Al Minufiya governorate, was carried out. During the last two years, 73 peach trees (cv. Florida grafted on Nemagardrootstock) were visually inspected and sampled. No symptom characteristic of PLMVd infection was observed. All samples were tested for the presence of PLMVd using molecular hybridization (Dot-blot) and reverse transcription-polymerasechain reaction (RT-PCR). Only one of the 73 peachtrees resulted positive for PLMVd as evidenced by Dot-blot and RT-PCR. Following RT-PCR amplification cloning and nucleotide sequence, the Egyptian PLMVd isolate showed an identity of 86 to 95% when compared with other PLMVd sequences in Genbank.

Czech name

Identifikace a molekulární charakterizace izolátu viroidu latentní mozaiky broskvoně infikujícího broskvoně v Egyptě

Czech description

In Egypt, a survey covering five commercial peach orchards of the newreclaimed lands, El Khatatba region in Al Minufiya governorate, was carried out. During the last two years, 73 peach trees (cv. Florida grafted on Nemagardrootstock) were visually inspected and sampled. No symptom characteristic of PLMVd infection was observed. All samples were tested for the presence of PLMVd using molecular hybridization (Dot-blot) and reverse transcription-polymerasechain reaction (RT-PCR). Only one of the 73 peachtrees resulted positive for PLMVd as evidenced by Dot-blot and RT-PCR. Following RT-PCR amplification cloning and nucleotide sequence, the Egyptian PLMVd isolate showed an identity of 86 to 95% when compared with other PLMVd sequences in Genbank.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

781

Country of publishing house

BE - BELGIUM

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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