Polyploidy induction in Phlox paniculata L. under in vitro conditions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F10%3A47321" target="_blank" >RIV/60460709:41210/10:47321 - isvavai.cz</a>
Alternative codes found
RIV/00027073:_____/10:#0000836
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Polyploidy induction in Phlox paniculata L. under in vitro conditions
Original language description
The objective of this work was to find an effective method of polyploidy induction using polyploidizating agents, colchicine and oryzalin, in diploid cultivar of Phlox paniculata 'Fujiyama' (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; polyploidizating agent infiltration from nutrient media ("the infiltration method") and dipping of the explants in a chemical mutagen solution ("the dip method"). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 ?mol l-1 oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
ISSN
1211-8516
e-ISSN
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Volume of the periodical
1
Issue of the periodical within the volume
2010
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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