The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F13%3A59827" target="_blank" >RIV/60460709:41210/13:59827 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/13:#0001782

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Original language description

Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granules (CG) exocytosis is still not fully understood. The aim of this study was to verify whether NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine(SNAP) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA), 1-oleyl-2-acetyl-sn-glycerol (OAG) and L-?-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP together with PKC-activators (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). The results showed that 0% of control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 ? 10

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GAP502%2F10%2F0794" target="_blank" >GAP502/10/0794: Acquisition of meiotic competence in pig oocytes - the effects of protein phosphatase calcineurin</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animal

ISSN

1751-7311

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

279-286

UT code for WoS article

000313218900012

EID of the result in the Scopus database

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