Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F14%3A64334" target="_blank" >RIV/60460709:41210/14:64334 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Original language description

The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50?g ml?1) and daidzein (DAI; 10, 20, 40, 50?g ml?1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20?g ml?1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 ?g ml?1.The cumulus expansion was also influenced in a different manner ? reduction of the COC?s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrat

Czech name

Biochanin A and daidzein influence meiotic maturation of pig oocytes in different manner

Czech description

The aim of the study was to determine the influence of different concentrations of phytoestrogens biochanin A (BIO A; 20, 40, 50?g ml?1) and daidzein (DAI; 10, 20, 40, 50?g ml?1) on the course of meiotic maturation of pig oocytes. After a 24-hour cultivation, a stage of nuclear maturation was achieved and the areas of cumulus-oocyte complexes (COCs), as an indicator of cumulus expansion, were evaluated. The effects of both contaminants on oocytes were mani - fested from the lowest concentration used. Nuclear maturation was inhibited in a dose-dependent manner in the case of BIO A. Effects of DAI reached a plateau at a concentration of 20?g ml?1.Possible relationship to limited solubility of DAI was excluded because limits of DAI solubility in culture medium were confirmed at 50 ?g ml?1.The cumulus expansion was also influenced in a different manner ? reduction of the COC?s area by BIO A was dose-dependent, whereas DAI had the strongest effect on CCs in the lowest and highest concentrat

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientia Agriculturae Bohemica

ISSN

1211-3174

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

155-161

UT code for WoS article

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EID of the result in the Scopus database

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