Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F16%3A71370" target="_blank" >RIV/60460709:41210/16:71370 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10330114
Result on the web
<a href="http://dx.doi.org/10.17221/27/2016-CJAS" target="_blank" >http://dx.doi.org/10.17221/27/2016-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/27/2016-CJAS" target="_blank" >10.17221/27/2016-CJAS</a>

Result language
angličtina
Original language name
Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results
Original language description
Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved as a cryoprotective agent, which can replace egg yolk in extenders. The aim of this study was, at first, to verify quality of produced LDL - newly preserved by sodium azide and especially to investigate the effects of LDL addition on cryoprotective properties of the soybean extenders. The quality of the LDL was verified using commercial egg yolk extender BULLXcell and concentrations of 6%, 8% and 10% LDL (v/v) as an egg yolk replacement. The effects of LDL addition to the soybean lecithin-based extenders in concentrations of 4%, 6% and 8% (v/v) were tested using extenders Andromed and Bioxcell. In total, 64 samples from eight bulls were evaluated. Kinematic parameters of spermatozoa, resulted from Computer Assisted Sperm Analysis, and their viability, evaluated by fluorescent technique, were assessed imm
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GI - Farm animal breeding and farm animal pedigree breeding
OECD FORD branch
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Project
<a href="/en/project/QJ1210109" target="_blank" >QJ1210109: Innovations of bull semen processing and technological procedures of AI dose manufacture leading to improvement of bulls´ inseminating capabilities</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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