Effect of Two Freezing Extenders on Characteristic of Fresh and Frozen-Thawed Semen in Endangered Old Kladruber Stallions - A Pilot Study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73482" target="_blank" >RIV/60460709:41210/17:73482 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/76/2016-CJAS" target="_blank" >http://dx.doi.org/10.17221/76/2016-CJAS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/76/2016-CJAS" target="_blank" >10.17221/76/2016-CJAS</a>
Alternative languages
Result language
angličtina
Original language name
Effect of Two Freezing Extenders on Characteristic of Fresh and Frozen-Thawed Semen in Endangered Old Kladruber Stallions - A Pilot Study
Original language description
The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots, these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserved insemination doses (IDs) from each extender (Gent and Lact) were prepared for each stallion from one collection (108 samples from six stallions in total). As a parameter of quality, the motility (computer assisted sperm analysis), viability (fluorescence staining), and morphology (eosin/nigrosine staining) were evaluated after dilution with freezing extenders (fresh) and after thawing (frozen-thawed. The diffe
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Animal Science
ISSN
1212-1819
e-ISSN
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Volume of the periodical
62
Issue of the periodical within the volume
6
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
227-233
UT code for WoS article
000403547200001
EID of the result in the Scopus database
2-s2.0-85020783738