Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73659" target="_blank" >RIV/60460709:41210/17:73659 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/389/2016-CJFS" target="_blank" >http://dx.doi.org/10.17221/389/2016-CJFS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/389/2016-CJFS" target="_blank" >10.17221/389/2016-CJFS</a>
Alternative languages
Result language
angličtina
Original language name
Validation of a UHPLC-ESI-MS/MS Method for Anthocyanidin Quantification in Potato Tubers
Original language description
The development of bioanalytical methods has become challenging due to sample complexity, requirements for method reliability, and speed of analysis with triple quadrupole LC-MS/MS used widely for the routine analysis of biological materials. The article presents the method development and validation results for pelargonidin and malvidin in potato tubers. The developed method uses a short C18 column, is able to measure all six common anthocyanidins, uses a binary mobile phase with acetonitrile and water both with added 1 per cent formic acid, ESI ionisation in positive mode,three hour hydrolysis with 2,7 M methanolic HCl at 90 degrees C. For pelargonidin and malvidin, the method shows high recovery of 98 to 100 per cent, intraday repeatability of 6,7 to 17,9 per cent (depending on the analyte and concentration level), uncertainty below 20 per cent, and uses quadratic calibration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21101 - Food and beverages
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Food Sciences
ISSN
1212-1800
e-ISSN
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Volume of the periodical
35
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
223-228
UT code for WoS article
000404566800006
EID of the result in the Scopus database
2-s2.0-85021663828