Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73828" target="_blank" >RIV/60460709:41210/17:73828 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17221/76/2015-CJGPB" target="_blank" >http://dx.doi.org/10.17221/76/2015-CJGPB</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/76/2015-CJGPB" target="_blank" >10.17221/76/2015-CJGPB</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of variations in plastid DNA non-coding regions in selected species of the genus Solanum
Original language description
The diversity of three non-coding plastid DNA loci (trnL/trnF spacer, trnV/16SrRNA spacer, trnL/trnL intron) was assessed in 16 Solanum L. species (135 individuals). Polymorphisms were detected by denaturing gradient gel electrophoresis (DGGE) and verified by direct sequencing. No intraspecific diversity and only poor interspecific diversity was detected. Unique S. mochiquense Ochoa specific length polymorphism at the trnL/trnL locus represented by duplication of an 18 bp segment was discovered. The detected DGGE interspecific trnL/trnF locus polymorphism did not specifically associate with single point mutations in the sequence confirmed by sequencing. The DGGE method was found to be a simple and cheap pre-exploring tool for mutation detection in compared DNA regions. Some identified polymorphisms can be used in the management of genetic resources.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
<a href="/en/project/QJ1210305" target="_blank" >QJ1210305: Integrated late blight management in new agro-environmental conditions using prediction of disease occurrence and based on new findings about pathogen population changes and tuber decay processes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Genetics and Plant Breeding
ISSN
1212-1975
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
5
Pages from-to
127-131
UT code for WoS article
000410679800006
EID of the result in the Scopus database
2-s2.0-85030125279