Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F17%3A73832" target="_blank" >RIV/60460709:41210/17:73832 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.anireprosci.2017.07.020" target="_blank" >http://dx.doi.org/10.1016/j.anireprosci.2017.07.020</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.anireprosci.2017.07.020" target="_blank" >10.1016/j.anireprosci.2017.07.020</a>
Alternative languages
Result language
angličtina
Original language name
Integrity of head and tail plasmalemma is associated with different kinetic variables in boar sperm
Original language description
An intact and functional sperm plasmalemma has a major role in sperm motility and fertilizing capacity. Several techniques have been developed to evaluate the integrity of the sperm plasma membrane, but there are still some inconsistencies concerning the methods that are more closely associated with sperm function. In this study, the aim was to: i) evaluate the integrity of the boar sperm plasmalemma during 72 h of semen storage at 17C using four techniques: eosin/nigrosin (E/N), propidium iodide/carboxyfluorescein diacetate (PI/CFDA), hypo-osmotic swelling test (HOST), and combined HOST with eosin staining (HOST/E), ii) assess the correlations and the limits of consistency among these techniques, iii) and estimate the relationships with the acrosomal status and sperm kinetics. Results indicate that the integrity of the sperm plasmalemma decreases during 72 h of storage, although significant differences were found only using the HOST and HOST/E techniques. Moreover, use of E/N and PI/CFDA results in
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal Reproduction Science
ISSN
0378-4320
e-ISSN
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Volume of the periodical
184
Issue of the periodical within the volume
N
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
218-227
UT code for WoS article
000411300700025
EID of the result in the Scopus database
2-s2.0-85026665960