CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76549" target="_blank" >RIV/60460709:41210/18:76549 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/18:00492845 RIV/00216208:11310/18:10382041
Result on the web
<a href="http://dx.doi.org/10.3390/ijms19041236" target="_blank" >http://dx.doi.org/10.3390/ijms19041236</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms19041236" target="_blank" >10.3390/ijms19041236</a>
Alternative languages
Result language
angličtina
Original language name
CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization
Original language description
Proteins CD9 and CD81 are members of the tetraspanin superfamily and were detected in mammalian sperm, where they are suspected to form an active tetraspanin web and to participate in sperm egg membrane fusion. The importance of these two proteins during the early stages of fertilization is supported by the complete sterility of CD9 CD81 double null female mice. In this study, the putative mechanism of CD9 CD81 involvement in tetraspanin web formation in sperm and its activity prior to fertilization was addressed. Confocal microscopy and colocalization assay was used to determine a mutual CD9 CD81 localization visualised in detail by super resolution microscopy, and their interaction was address by co-immunoprecipitation. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. During the acrosom
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
1-17
UT code for WoS article
000434978700315
EID of the result in the Scopus database
2-s2.0-85045909159