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Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A78011" target="_blank" >RIV/60460709:41210/18:78011 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/18:00506670

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s00441-017-2705-x" target="_blank" >http://dx.doi.org/10.1007/s00441-017-2705-x</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00441-017-2705-x" target="_blank" >10.1007/s00441-017-2705-x</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

  • Original language description

    Phosphorylation, or dephosphorylation, is one of the most frequent posttranslational modifications regulating protein protein activity in eukaryotic cells. Whereas mature spermatozoa (as specialized cells) are transcriptionally inactive and do not synthesize new proteins, phosphorylation of sperm proteins is very important for the regulation of the sperm function. Although the posttesticular maturation of spermatozoa is a process common to all mammals, comparative studies showed significant differences in sperm surface proteins and the mechanisms of protein modification during the epididymal maturation. In our study, the evaluation of tyrosine phosphorylation, represented by the fluorescent patterns of used antiphosphotyrosine antibodies (P Tyr 01 and 4G10), in spermatozoa isolated from different regions of the epididymis caput, corpus and cauda was performed. Although in general both antibodies detected almost the same reaction patterns, we observed some dissimilarity associated with the binding spe

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10604 - Reproductive biology (medical aspects to be 3)

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    CELL AND TISSUE RESEARCH

  • ISSN

    0302-766X

  • e-ISSN

    1432-0878

  • Volume of the periodical

    371

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

    365-373

  • UT code for WoS article

    000423324400013

  • EID of the result in the Scopus database

    2-s2.0-85031929786