Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F18%3A00506670" target="_blank" >RIV/86652036:_____/18:00506670 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/18:78011

Result on the web

<a href="https://link.springer.com/article/10.1007/s00441-017-2705-x" target="_blank" >https://link.springer.com/article/10.1007/s00441-017-2705-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00441-017-2705-x" target="_blank" >10.1007/s00441-017-2705-x</a>

Result language

angličtina

Original language name

Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis

Original language description

Phosphorylation, or dephosphorylation, is one of the most frequent post-translational modifications regulating protein-protein activity in eukaryotic cells. Whereas mature spermatozoa (as specialized cells) are transcriptionally inactive and do not synthesize new proteins, phosphorylation of sperm proteins is very important for the regulation of the sperm function. Although the post-testicular maturation of spermatozoa is a process common to all mammals, comparative studies showed significant differences in sperm surface proteins and the mechanisms of protein modification during the epididymal maturation. In our study, the evaluation of tyrosine phosphorylation, represented by the fluorescent patterns of used anti-phosphotyrosine antibodies (P-Tyr-01 and 4G10), in spermatozoa isolated from different regions of the epididymis caput, corpus and cauda was performed. Although in general both antibodies detected almost the same reaction patterns, we observed some dissimilarity associated with the binding specificity of the antibodies and also the segment-dependent manner of phosphorylated protein localization. These data were filled up by immunohistochemical analysis of testes and epididymides cryosections. Additionally, our phosphoproteomic study focused on evaluation of the changes in the pattern of tyrosine-phosphorylated proteins during the post-testicular maturation of bull spermatozoa (PY20 antibody). To summarize the results, an increasing trend of tyrosine phosphorylation of proteins during the maturation of bull sperm in the epididymis was consistently observed in all the methods/experiments.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell and Tissue Research

ISSN

0302-766X

e-ISSN

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Volume of the periodical

371

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

365-373

UT code for WoS article

000423324400013

EID of the result in the Scopus database

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