Complexity and modification of the bull sperm glycocalyx during epididymal maturation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F24%3A00586446" target="_blank" >RIV/86652036:_____/24:00586446 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/24:98491
Result on the web
<a href="https://faseb.onlinelibrary.wiley.com/doi/epdf/10.1096/fj.202400551RR" target="_blank" >https://faseb.onlinelibrary.wiley.com/doi/epdf/10.1096/fj.202400551RR</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.202400551RR" target="_blank" >10.1096/fj.202400551RR</a>
Alternative languages
Result language
angličtina
Original language name
Complexity and modification of the bull sperm glycocalyx during epididymal maturation
Original language description
Mammalian spermatozoa have a surface covered with glycocalyx, consisting of heterogeneous glycoproteins and glycolipids. This complexity arises from diverse monosaccharides, distinct linkages, various isomeric glycans, branching levels, and saccharide sequences. The glycocalyx is synthesized by spermatozoa developing in the testis, and its subsequent alterations during their transit through the epididymis are a critical process for the sperm acquisition of fertilizing ability. In this study, we performed detailed analysis of the glycocalyx on the sperm surface of bull spermatozoa in relation to individual parts of the epididymis using a wide range (24) of lectins with specific carbohydrate binding preferences. Fluorescence analysis of intact sperm isolated from the bull epididymides was complemented by Western blot detection of protein extracts from the sperm plasma membrane fractions. Our experimental results revealed predominant sequential modification of bull sperm glycans with N-acetyllactosamine (LacNAc), followed by subsequent sialylation and fucosylation in a highly specific manner. Additionally, variations in the lectin detection on the sperm surface may indicate the acquisition or release of glycans or glycoproteins. Our study is the first to provide a complex analysis of the bull sperm glycocalyx modification during epididymal maturation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA22-31156S" target="_blank" >GA22-31156S: Key molecules involved in gamete maturation and sperm-zona pellucida recognition in pig and cattle</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FASEB Journal
ISSN
0892-6638
e-ISSN
1530-6860
Volume of the periodical
38
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
23
Pages from-to
e23687
UT code for WoS article
001230210500001
EID of the result in the Scopus database
2-s2.0-85194127693