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Estimation of genotyping errors of STR markers in dogs and wolves

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A80820" target="_blank" >RIV/60460709:41210/19:80820 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.fsigss.2019.11.014" target="_blank" >10.1016/j.fsigss.2019.11.014</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Estimation of genotyping errors of STR markers in dogs and wolves

  • Original language description

    This article will discuss the estimation of genotyping errors for Mini-DogFiler panel A and B in group of wolfs (n = 39) where the source material for DNA isolation were faeces and control group of dogs (n = 170), represented by Czechoslovakian wolfdog breed (CSW), where DNA was obtained from buccal swabs. DNA samples isolated from buccal swabs had a very low frequency of genotyping error estimated as mean error rate per allele (ea = 0,09%), mean error per locus (el = 0,18%) and error rate per multilocus genotype (eobs = 2,17%). Completely different situation was observed for DNA samples isolated from faeces and affirms how complicated is to get reliable results from such material. Total performance was very poor where only 7 out of 39 samples replicated (about 18%) at least 2 times out of 4 replicates. In such case would be eobs = 86,5%. For this type of samples is typical very fragmented DNA with low concentration. This fact leads to the occurrence of many of negative effects like an allelic dropou

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30501 - Forensic science

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Forensic Science International: Genetics Supplement Series

  • ISSN

    1875-1768

  • e-ISSN

    1875-175X

  • Volume of the periodical

    7

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    2

  • Pages from-to

    901-902

  • UT code for WoS article

    000508217000344

  • EID of the result in the Scopus database

    2-s2.0-85076235622