Estimation of genotyping errors of STR markers in dogs and wolves
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F19%3A80820" target="_blank" >RIV/60460709:41210/19:80820 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1875176819300800?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fsigss.2019.11.014" target="_blank" >10.1016/j.fsigss.2019.11.014</a>
Alternative languages
Result language
angličtina
Original language name
Estimation of genotyping errors of STR markers in dogs and wolves
Original language description
This article will discuss the estimation of genotyping errors for Mini-DogFiler panel A and B in group of wolfs (n = 39) where the source material for DNA isolation were faeces and control group of dogs (n = 170), represented by Czechoslovakian wolfdog breed (CSW), where DNA was obtained from buccal swabs. DNA samples isolated from buccal swabs had a very low frequency of genotyping error estimated as mean error rate per allele (ea = 0,09%), mean error per locus (el = 0,18%) and error rate per multilocus genotype (eobs = 2,17%). Completely different situation was observed for DNA samples isolated from faeces and affirms how complicated is to get reliable results from such material. Total performance was very poor where only 7 out of 39 samples replicated (about 18%) at least 2 times out of 4 replicates. In such case would be eobs = 86,5%. For this type of samples is typical very fragmented DNA with low concentration. This fact leads to the occurrence of many of negative effects like an allelic dropou
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30501 - Forensic science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Forensic Science International: Genetics Supplement Series
ISSN
1875-1768
e-ISSN
1875-175X
Volume of the periodical
7
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
2
Pages from-to
901-902
UT code for WoS article
000508217000344
EID of the result in the Scopus database
2-s2.0-85076235622